Monday, October 10, 2016

Egg Macromolecule Lab

In this lab we asked the question can macromolecules be identified in an egg cell. We found that the egg membrane have proteins, which had a 10 on our color scale, which was from 0 to 10, 0 being no color change and 10 being a very dark color change. The reason for proteins to be found in the membrane is because the egg membrane represents the cell membrane, which has protein channels spread throughout the cell membrane, letting bigger molecules in and out of the cell, and thus, the sodium hydroxide and copper sulfate mixture detected them. We found that the egg yolk had lipids, which had a 9. The reason for lipids were found in the yolk is because the egg yolk represents nuclei of a cell, which has a nucleic membrane made of lipids, thus the egg yolk would contain some lipids and would be detected by Sudan 3. We found the egg white contains proteins, which has a 10. The reason for proteins to be located in the egg white is because the egg white represents the cell's cytoplasm, which contains many organelles. Some organelles are used to create proteins, and have proteins inside of them going through process, so that explains why proteins are found in the egg white and why they were detected by the sodium hydroxide and copper sulfate mixture.

Our data for the egg membrane supports our hypothesis, if the egg membrane has monosaccharides and polysaccharides, then it will turn from blue to green or orange for monosaccharides, which gave us a result of 8, and brown to black for polysaccharides, which gave us a 3. But there could have been some errors because the egg membrane might have been accidentally touched by human hands. Our data for the egg yolk supports our hypothesis, if the egg yolk has lipids in it, it will turn from red to orange, which gave us a result of 9. But there could have been some errors because maybe the same amount of solution wasn't used for each test tube. Our data for the egg white supports the protein part of our hypothesis and disapproves the lipid part of our hypothesis. Our hypothesis states that if the egg white has lipids and proteins, it will turn from red to orange for lipids, which gave us a 0, and from blue to purple for proteins, which was a 10. Some errors that could have happened is the data may have been recorded wrong for color change, like a slight change of color wasn't given a 1 or 2, but a 0. Due to these errors, in future experiments I would recommend to try to be more precise with measuring, being more observative, and trying to contaminate things.

This lab was done to demonstrate the molecules that can be found in an egg cell. From this lab I have learned that types of macromolecules can be found in certain parts of a cell which helps me understand the concept of were certain macromolecules are located in the cell.  Based on my experience from this lab I can understand what certain types of food have certain molecules.

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